Laser-assisted fabrication and in vitro verification of functionalized surface for cells biointegration

The paper investigates how the surface relief of an implant affects cell behavior. Currently, most manufacturers claim key impact biocompatibility factor to be micro-roughness. We suppose that interaction between cells and implants also depends on such peculiarities as continuous or discontinuous topography, subcellular distance peaks presence porous oxide layer. have developed laser processing conditions provide three different reliefs: «open grooves», «grid» «close grooves». Along with micro-roughness characteristics reliefs differ their deepness period grooves. composition analysis results shown a sandwich structure consisting Ti → TiO Ti2O3Nx TiO2 (anatase) (rutile). wettability study has demonstrated superhydrophilicity (CA is 0°) for all reliefs. quantitative qualitative hMSCs proliferation osteogenic differentiation was performed 20 days. In vitro revealed topography spatial orientation cells. shape size nuclei vary topographies. found grooves» structures cellular are beneficial cells' life-sustaining activity. Discontinuous individual slots might not mobility least external mechanical effect compared